This book badly needs revision | Unraveling DNA: Molecular Biology for the Laboratory | Michael R. Winfrey, Marc A. Rott, ...
 
 


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Unraveling DNA: Molecular Biology for the Laboratory
Michael R. Winfrey, Marc A. Rott, ...

Benjamin Cummings, 1997 - 400 pages

average customer review:based on 3 reviews
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Two thumbs up

One of the best lab texts around. Provides a thorough exposure to the most commonly used molecular laboratory techniques. Highly recommended for faculty, who focus on creating a hands-on learning environment.


Wonderful Lab Manual

This is a wonderful text clear and concise, it explores the bulk of the basic lab techniques and procedures in molecular biology. This highly readable text builds on the cumulative experiences of some highly skilled academicians and researchers. Highly recommended for any molecular biology lab course or as a useful reference.


This book badly needs revision

The material in the introductory text preceeding most of the exercizes lacks clarity, is poorly written and sadly confuses the reader. The introduction to the lux operon seems to be a very good example to illustrate the point. This book was made possible by a grant from the NSF and if the authors continue to market this book without revision it would be just be a travesty and an insult to intelligence. Please take note of the shortcomings of this book before decinding to buy it.


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This manual encompasses an integrated series of molecular biology laboratory exercises that involve the cloning and analysis of the bioluminescence (lux) genes from the marine bacterium Vibrio fischeri. The manual is divided into discrete units with each demonstrating one or more aspects of the cloning project. The manual is based on one of nature's most fascinating biological phenomenon: the biological production of light. This results in a recurrent theme of interest and makes the project very relevant to interdisciplinary topics such as fish symbiosis, biochemistry, biophysics, etc. Includes instruction in the basic techniques of modern molecular biology: DNA isolation and analysis, DNA restriction, agarose gel electrophoresis, ligations, transformation of recombinant DNA, preparation and screening a genomic library, restriction mapping, Southern blotting, hybridization, DNA sequencing, pulsed field gel electrophoresis. Designed for a one semester course in Molecular Biology. Also appropriate for a molecular biology component of Microbial Genetics, Genetics, Biochemistry, or Advanced Microbiology courses.


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